Smart Computing- Book ISBN- 978-81-953658-1-4

Authors:- Rohit Lade, Arun Arya, Manis Lenka, Shivam Barthwal

Abstract :-The present study is aimed at biodegradation of 4-Chlorophenol and 2,4-Dichlorophenol using Pseudomonas aeruginosa and Bacillus subtilis in the form of mixed and pure cultures. Optimum degradation conditions of 30°C temperature and 7 pH for maximum removal rate of chlorophenols have been established in this study. The mixed culture consisting of 50/50 Pseudomonas aeruginosa and Bacillus subtilis cultures shows maximum chlorophenol removal efficiency as compared to pure culture. After about 130 hours of contact, mixed culture could degrade the chlorophenols completely. Finally considering the difficulties related to segregating pure cultures and then using them for degradation, the use of mixed culture for chlorophenols removal has been proposed.

Keywords: - Biodegradation; 4-Chlorophenol; 2,4-Dichlorophenol; Pseudomonas aeruginosa; Bacillus subtilis.