Smart Computing- Book ISBN- 978-81-953658-1-4

Authors:- Diksha Saxena, Vishal Kumar Sandhwar, Shivendu Saxena

Abstract :-Textile wastewater contaminated with dye and soil samples were collected from a small scale industry located at Bhadohi, Uttar Pradesh, India. Study was conducted for screening and isolation of bacteria capable for decolorizing textile dyes. Reactive Black 5 and Reactive Blue 4 dyes were used in the study. Decolorization abilities of isolates were studied and optimizations of various physiochemical parameters (effect of pH, contaminant concentration, inoculum concentration) were examined. Products after dye degradation were analyzed by UV-VIS techniques. Bacterial identification tests (Gram Staining Test, Indole Test, Methyl Red Test, Voges-Proskauer Test, Citrate Utilization Test) were conducted in order to find the family of bacteria. Results show that the optimum pH for dye decolorization was 7.0 for 10, 15 and 20 ppm concentration, Reactive Blue 4 at 10 ppm concentration was showing maximum decolorization after 36 hours, 14 ml inoculum was showing maximum decolorization for Reactive Black 5 and Reactive Blue 4 dyes after 48hours.

Keywords: - Bioremediation, Textile wastewater, Gram Staining Test, Indole Test, Methyl Red Test, Voges-Proskauer Test, Citrate Utilization Test.