Authors: Het Patel, Prof, Mitali Dalwadi, Dr. U.M.UpadhyayÂ
Abstract: Chromatography is defined as a set of techniques which is used for the separation of constituents in a mixture. This technique involves 2 phases stationary and mobile phases. The separation of constituents is based on the difference between partition coefficients of the two phases. The chromatography term is derived from the Greek words, namely chroma (colour) and graphein (to write). Chromatography is a very popular technique and it is mostly used analytically. There are different types of chromatographic techniques, namely Paper Chromatography, Gas Chromatography, Liquid Chromatography, Thin Layer Chromatography (TLC), Ion exchange Chromatography and lastly, High-Performance Liquid Chromatography (HPLC). This review mainly focuses on the HPLC technique, its principle, types, instrumentation and applications. High-performance liquid chromatography is the most accurate method extensively used for the qualitative and quantitative analysis of drug products. Analytical method development and validation play vital role in the drug discovery, Drug development and manufacture of pharmaceuticals. A simple, precise, accurate, specific and RP-HPLC method was developed for the determination of drugs in pharmaceutical formulation.
Keywords: HPLC, RP-HPLC, Chromatography, Chromatographic Conditions, Abacavir (ABAC), Lamivudine (LAMI).
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