Journal of Pharmacovigilance and Quality Assurance ISSN: 3108-2343 (Online)

Authors: Sowjanya Gummadi, Meghasesha Myneni

Abstract: Two simple, economical and reproducible fundamental and derivative UV spectrophotometric methods were developed and validated for determination of Famotidine in bulk and dosage form. Famotidine showed maximum absorption at 281 nm in phosphate buffer pH 7.5 while it has 282 nm as its absorption maxima in borate buffer pH 9.0. The linearity was determined in the concentration range of 30-80 μg/mL (r2 as 0.9993 & 0.9987) and 10-60 µg/mL (r2 as 0.9991 & 0.9995) for the fundamental and derivative methods in phosphate and borate buffers. The developed methods were validated as per ICH guidelines. Recovery studies gave satisfactory results indicating that none of the major additives/excipients interfered with the assay method. This method may be useful for routine laboratory analysis of famotidine.

Keywords: Borate buffer pH 9.0, derivative, famotidine, fundamental, phosphate buffer pH 7.5, UV spectrophotometric, validation