Author: Dhruta R Parikh
Abstract: Clobetasol propionate Samples were diluted with Sodium dihydrogen phosphate monohydrate: methanol: acetonitrile (30:20:50). The chromatographic conditions were set, at a flow rate of 1.0ml/min giving retention times of 4 to 8 minutes (at least 1.2 times of retention time of analyte) for Clobetasol Propionate. The sample injection volume was 20µL and the detection was set at a wavelength of 272nm (UV-Vis detector). Validation was carried out using pure standards of Clobetasol propionate to achieve good linearity at a concentration range of between 95% to 105.0% with a detection limit for Clobetasol Propionate. The internal standard method reported slight improvement of the confidence limit and the relative standard deviation relative to the external standard method. HPLC method has been used to analyse Clobetasol propionate.
Keywords: Analytical method development, Validation, Clobetasol propionate
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