Authors: Dr. Priyanka Joshi, Mr. Aditya Singh
Abstract: Accurate quantification of drugs in biological fluids is crucial for pharmacokinetic, pharmacodynamic, and therapeutic drug monitoring studies. Bioanalytical techniques have evolved to provide high sensitivity, selectivity, and reproducibility, enabling precise measurement of drug concentrations in complex biological matrices such as plasma, serum, urine, and tissues. Techniques such as High-Performance Liquid Chromatography (HPLC), Liquid Chromatography-Mass Spectrometry (LC-MS/MS), Gas Chromatography (GC), and immunoassays are extensively employed in drug quantification. This paper provides an overview of bioanalytical techniques, focusing on their principles, applications, sample preparation strategies, method validation, and regulatory compliance. The integration of hyphenated techniques and automation has enhanced throughput, sensitivity, and accuracy. Implementing these bioanalytical methods is vital for ensuring drug safety, efficacy, and regulatory approval in both preclinical and clinical studies.
Keywords: Bioanalytical Techniques, Drug Quantification, HPLC, LC MS/MS, Biological Fluids, Pharmacokinetics, Method Validation
Full Issue
| View or download the full issue | PDF 131-136 |