Authors : Dr. Karan Mehta, Ms. Anjali Rao
Abstract: Chromatography plays a critical role in drug discovery and development, offering powerful techniques for separation, identification, and quantification of pharmaceutical compounds. Its applications range from initial lead compound screening to formulation analysis, impurity profiling, and pharmacokinetic studies. Techniques such as High-Performance Liquid Chromatography (HPLC), Gas Chromatography (GC), Ultra-Performance Liquid Chromatography (UPLC), and capillary electrophoresis provide high resolution, sensitivity, and reproducibility essential for modern drug development. This paper reviews the fundamental principles of chromatographic techniques, recent technological advancements, and their integration with analytical and computational tools. Emphasis is placed on method optimization, validation, and application in regulatory compliance. The implementation of advanced chromatography enhances the efficiency of drug development pipelines, ensures product quality, and accelerates approval processes. Furthermore, the integration of hyphenated techniques and chemometrics improves structural elucidation, impurity detection, and pharmacokinetic analysis, highlighting the indispensable role of chromatography in pharmaceutical sciences.
Keywords: Chromatography, HPLC, UPLC, Drug Discovery, Impurity Profiling, Pharmacokinetics, Pharmaceutical Analysis
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