Application of Chromatography in Pharmaceutical Drug Analysis

Abstract

Chromatography remains a cornerstone of pharmaceutical analysis due to its superior separation, identification, and quantification capabilities. Among the chromatographic techniques, High-Performance Liquid Chromatography (HPLC), Gas Chromatography (GC), and Thin Layer Chromatography (TLC) are widely used for purity assessment and stability studies. This paper presents an overview of chromatographic principles, instrument configurations, and method development strategies applied in pharmaceutical research. Advanced hybrid systems such as LC-MS and GC-MS have enabled trace-level impurity profiling and complex mixture analysis. Furthermore, green chromatography, employing eco-friendly solvents and sustainable materials, is emerging as a critical focus area. The study also discusses method validation parameters such as linearity, sensitivity, and reproducibility. Chromatography continues to evolve as an indispensable analytical tool in pharmaceutical quality assurance and research.

KEYWORDS: - Chromatography, HPLC, GC-MS, Method Validation, Green Chemistry